CHAPS, Zwitterionic Non-Denaturing Detergent, Ultra Pure, ≥98%

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) is a zwitterionic, non-denaturing detergent derived from cholic acid. It solubilizes membrane proteins and disrupts protein–protein interactions while preserving native structure and activity. Its net-neutral charge makes it compatible with isoelectric focusing and ion-exchange chromatography.
 
Key features
Zwitterionic: net-neutral charge over a wide pH range
Non-denaturing: preserves protein structure and enzyme activity
High critical micelle concentration (6–10 mM), so it is easily removed by dialysis
Small micelles (about 6 kDa) and low UV absorbance at 280 nm
Ultra pure grade, ≥98%
 
Applications
Solubilization of membrane proteins and receptors
Sample and rehydration buffers for isoelectric focusing and 2D gels (typically 2–4%)
Lysis buffers for immunoprecipitation and co-immunoprecipitation
Reducing non-specific binding in protein assays
 
Specifications
CAS number: 75621-03-3
Molecular formula: C32H58N2O7S
Molecular weight: 614.88 g/mol
Detergent type: Zwitterionic, non-denaturing
Critical micelle concentration: 6–10 mM
Purity: ≥98%
Grade: Ultra pure
Appearance: White powder
Storage: 2–8 °C
 
Ordering information
1 g: SKU TQBLNB010-1
5 g: SKU TQBLNB010-5
10 g: SKU TQBLNB010-10
25 g: SKU TQBLNB010-25
 
For research use only.