PuroSPIN™ Fibrous Tissue RNA Purification Kit is designed for extraction of total RNA from fibrous animal tissue, such as skin, skeletal muscle, lung, or heart. It greatly simplifies the extraction process by incorporating a convenient easy-to-use extraction technology based on nucleic acid binding silica spin-columns. The tissue is first disrupted through homogenization and Proteinase K treatment. The RNA is then released form the cells by the treatment with the Lysis Buffer LB-R. Following the lysis step, RNA is loaded onto silica-based spin columns and washed to remove protein, salt and other contaminants. The kit includes on-column DNase I digestion step to remove any contaminating DNA. Finally, purified RNA is eluted from the column into a clean microcentrifuge tube in Elution Buffer EB, or nuclease-free water.
The kit can also be used to perform RNA cleanup after DNase I or other enzymatic treatments. High quality RNA extracted with the PuroSPIN™ Fibrous Tissue RNA Purification Kit can be used for a range of downstream applications, such as reverse-transcription PCR or qPCR, northern blotting, microarray analysis.
For purification of RNA from soft tissue, such as spleen, kidney, liver, or brain, or from bacteria, yeast, or mammalian cells, please use our PuroSPIN™ Total RNA Purification Kit (#NK051-50, #NK051-250).
PuroSPIN™ columns can purify up to 200 μg of RNA product.