Human Epidermal Growth Factor (EGF), Dye Labeled, 100 µg

Recombinant Human Epidermal Growth Factor (EGF) that has been covalently labeled with a fluorescent dye.
 
The following fluorescence dye labels are available:
AFDye 488
AFDye 555
AFDye 647
IR750
 
NOTE 1: AFDye 488 (similar to FITC), AFDye 555 (similar to Cy3), AFDye 647 (similar to Cy5) are analogs of AlexaFluor® 488, AlexaFluor® 555, and AlexaFluor® 647 dyes, respectively.
NOTE 2: IR750 dye is ithe analog of the IRDye® 750 dye.
 
Epidermal Growth Factor (EGF) is an acidic, 6,200 Dalton peptide (53 amino acids) with 6 conserved cysteine motifs that form three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens.EGF is one of the most stable proteins, with great tolerance to acid, alkali, heat and enzymolysis of trypsin, chymotrypsin and pepsin. EGF binds EGFR on cell membrane and stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells. Recombinant Epidermal Growth Factor (Rh-EGF) is manufactured by the extracellular secretion and is structurally homologous to human native EGF with complete biolog ical activity.
 
Applications
EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells.
Proliferation and differentiation of a different cell types deriving from ectoderm and mesoderm.
 
Activity
1000000 IU / mg
 
Theoretical pI
5.20
 
Formulation
Lyophilized powder
 
Molecular Weight
6.2 kDa
 
Purity
≥95% by SDS-PAGE and SEC-HPLC analysis
 
Source
Escherichia coli
 
Storage Condition
-20ºC