NEST BioFactory™: 40 Chambers, 2 Narrow Mouth Caps, TC, Sterile, 2 Pcs/Cas
NEST BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications such as production of vaccines, monoclonal antibodies or pharmaceuticals. They’re versatile and easy-to-use systems for medium to large scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions.
Packaging: Double Bag Package, 1 pc/pack, 2 packs/case, 2 pcs/case. 4 Sterile Vented Overcaps Included Separately
Available in five sizes:
• 1-Chamber with 647 cm2 cell growth area
• 2-Chamber with 1279 cm2 cell growth area
• 5-Chamber with 3175 cm2 cell growth area
• 10-Chamber with 6335 cm2 cell growth area
• 40-Chamber with 25295 cm2 cell growth area
- Made of high clarity, 100% virgin polystyrene.
- Sterilized by E-beam, SAL=10-6.
- Non-Pyrogenic, DNase/Rnase free.
- Individually packaged in double-layer sterile bags.
- TC treated, good for cell attachment and growth.
- Clear lot number for batch traceability.
- Ultrasonic welding without extrinsic ingredients.
- Excellent welding line design can avoid producing particles during welding and make the biofactory stronger (Under Features 7th line).
- Vent caps with 0.22 μm hydrophobic filters for gas exchange without contamination.
- Wide mouth is designed for pouring culture medium directly. Narrow mouth is designed for operation with the aseptic pipeline.
- Growth kinetics are the same as cells grow in the cell culture flasks. Applicable to large-scale culture adherent cells.
- Large growing area. Just a single operate, you can cultivate a large number of cells and reduce contamination risk.
- Adaptable for automation.
- Standardized operations to reduce batch differences.
SKU: 772403
Product Data Sheet
Protocol
NOTE: See the visual diagram in the product images section
- Mix cell suspension with medium: Prepare cell suspension of required concentration in a container. Recommended volume is about 30- 50mL per layer.
- Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foam and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. (Save a little
- liquid in pipette each time.)
- Tips: A 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo.
- Hold the Multi-layer Flask upright with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer.
- While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up.
- After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces.
- Tips: Be careful to avoid foaming of medium, and not to spill liquid from each layer.
- Repeat Step 3 to put the flask quickly and slightly into the incubator . Then, lay it flat as shown in Step 4. You may choose to either aspirate or pour the media from Multi-layer Flask.
- Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media.
- Pouring method: With Logo facing you, pour spent media from Multi-layer Flask
- Tips: Aspirate media using a NEST 2 mL or 10mL aspirating pipette.
- Wash with buffer for one time and add dissociating reagent (≥5mL per layer). Then, follow Steps 3-4 to distribute to dissociating reagent to each layer.
- Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely.
- Follow Step 7 “Aspirating Method” protocol and collect cell suspension using a NEST 10mL serological pipette.
- Follow Step 8 “Pouring Method”. Pour the cell suspension into a NEST conical tube.
- Rinse with additional wash buffer if needed.
