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Streptavidin Coated Magnetic Beads, 500 nm Diameter

Streptavidin Coated Magnetic Beads, 500 nm Diameter

500 nm diameter magnetic beads with Streptavdin protein functionalized surface.

 

Features

  • High binding capacity of Biotin-conjugated molecules:
    • 2,000 to 4,000 pmole of biotin / mg of magnetic beads
    • 15-20 µg botinylated antibody / mg of magnetic beads
  • Rapid and efficient biomolecule purification from complex samples.
  • Fast response to a magnet.
  • High monodispersity.
  • Stable in high salt ­­conditions.

 

Applications

  • Magnetic separation of Biotinylated cells, DNA, proteins, antibodies, and small ligands from complex samples. The streptavidin-coated magnetic beads are simply added to Biotinylated molecules for binding. The samples are transferred into a magnetic rack for easy removal of unbound residue.

 

Citations:

  1. J. H. Molinski, S. Parwal and J. X. J. Zhang, "Laser-Manufactured Magnetic Microchips for Exosome Isolation and Patterning," 2024 IEEE 37th International Conference on Micro Electro Mechanical Systems (MEMS), Austin, TX, USA, 2024, pp. 336-339, doi: 10.1109/MEMS58180.2024.10439482.
  2. Molinski, John H., Siddhant Parwal, and John XJ Zhang. "Laser‐Patterning of Micromagnets for Immuno‐Magnetophoretic Exosome Capture." Small Methods (2024): 2400388.

 

  • Protocols

    Protocol A: Immobilization of proteins / antibodies

    • Vortex PuroMAG™ magnetic beads before use to fully resuspend the beads.
    • Transfer the desired volume of beads into a new 1.5 mL microcentrifuge tube.
    • Add biotinylated protein / antibody in 1X PBS to the tube. Incubate for 30 min at room temperature using gentle rotation.
    • Place the beads on the magnetic rack and incubate for 2 min. Remove the supernatant.
    • Remove the tube from the rack, add 1 mL of Wash Buffer PR and mix well. Place the tube on the magnetic rack and incubate for 2 min. Remove the supernatant.
    • Repeat washing Step 5 two more times.
    • Resuspend in the desired amount of 1X PBS.

    NOTE: If a significant amount of non-specific adsorption of proteins / antibodies to the beads is observed, pre-wash the beads in Wash Buffer PR before performing protein / antibody immobilization:

    • Place the beads on the magnetic rack and incubate for 2 min. Remove the supernatant. Resuspend in the volume of Wash Buffer PR equal to the volume of stock beads used.

     

    Protocol B: Immobilization of nucleic acids

    • Vortex PuroMAG™ magnetic beads before use to fully resuspend the beads.
    • Transfer the desired volume of beads into a new 1.5 mL microcentrifuge tube.
    • Place the tube on the magnetic rack and incubate for 2 min. Discard the supernatant.
    • Add the same volume of Wash Buffer NA, 2X as the volume of stock beads used.
    • Add an equal volume of biotinylated DNA or RNA nucleic acid resuspended in ultra-pure water.
    • Incubate for 15 min at room temperature using gentle rotation.
    • Place the beads on the magnetic rack and incubate for 2 min. Remove the supernatant.
    • Remove the tube from the rack, add 1 mL of Wash Buffer NA, 1X and mix well. Place the tubes on the magnetic rack and incubate for 2 min. Remove the supernatant.
    • Repeat washing Step 8 two more times.
    • Resuspend the washed beads in the desired amount of 1X PBS or alternative buffer appropriate for the downstream application.

     

    Protocol C: Releasing Immobilized Biotinylated Proteins / Antibodies

    • Boil the samples for 5 min in 0.1% SDS. This will denature the proteins, including streptavidin, and will release biotinylated molecules into the solution.

     

    Protocol D: Releasing Immobilized Biotinylated DNA

    • Incubate the beads for 5 min at 65°C (or 2 min at 90°C) in 10 mM EDTA with 95% formamide.
$245.00Price

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