PuroSPIN-96™ Gel Extraction Kit #NKP107-384

The PuroSPIN™ 96 Gel Extraction Kit is designed to provide a simple and easy-to-use method for extraction and purification of DNA samples (100 bp − 10 kbp) from agarose gels.
 
This purification technology is based on dissolving the gel embedded DNA in a buffer solution, followed by nucleic acids binding to 96-well silica membrane purification plates, which allows for efficient removal of gel residues and other contaminants. Purified DNA is eluted from the silica column into a clean 96-well collection plate in Elution Buffer EB, or nuclease-free water.
 
Silica membrane plates provide a faster, simpler, and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
 
The PuroSPIN™ buffer formulations produce highly purified DNA for downstream applications such as PCR, restriction-enzyme digestion, ligation, labeling, or sequencing. The PuroSPIN™ columns can bind up to 200 µg of DNA product.
 
NOTE: Our Universal Vacuum Manifold for 96-Well Plates (https://www.lunanano.ca/product-page/universal-vacuum-manifold-for-96-well-plates), or Double-Layer Vacuum Manifold for 96-Well Plates (https://www.lunanano.ca/product-page/double-layer-vacuum-manifold-for-96-well-plates) CAN ONLY be used with Semi-Skirted 96-well Nucleic Acid Purification Plate. 
 
Features:
Rapid purification of DNA fragments from standard agarose gels or polyacrylamide (PAGE) gels
High throughput sample processing using 96-well silica membrane purification plates
Simple extraction process with no need for phenol or chloroform
Efficient removal of dNTPs, primers, enzymes, and salts from PCR reaction mixtures and gel mixtures
High quality extracted DNA can be used for a range of downstream applications such as sequencing, ligation, transformation, transfection, Southern blotting, qPCR, or microarray analysis
 
SKU List: NKP107-384, NKPS107-384