PuroSPIN-96™ Gel Extraction Kit #NKP107-384
The PuroSPIN™ 96 Gel Extraction Kit is designed to provide a simple and easy-to-use method for extraction and purification of DNA samples (100 bp − 10 kbp) from agarose gels.
This purification technology is based on dissolving the gel embedded DNA in a buffer solution, followed by nucleic acids binding to 96-well silica membrane purification plates, which allows for efficient removal of gel residues and other contaminants. Purified DNA is eluted from the silica column into a clean 96-well collection plate in Elution Buffer EB, or nuclease-free water.
Silica membrane plates provide a faster, simpler, and safer alternative to traditional nucleic acid purification techniques such as phenol-chloroform extraction.
The PuroSPIN™ buffer formulations produce highly purified DNA for downstream applications such as PCR, restriction-enzyme digestion, ligation, labeling, or sequencing. The PuroSPIN™ columns can bind up to 200 µg of DNA product.
Features:
- Rapid purification of DNA fragments from standard agarose gels or polyacrylamide (PAGE) gels
- High throughput sample processing using 96-well silica membrane purification plates
- Simple extraction process with no need for phenol or chloroform
- Efficient removal of dNTPs, primers, enzymes, and salts from PCR reaction mixtures and gel mixtures
- High quality extracted DNA can be used for a range of downstream applications such as sequencing, ligation, transformation, transfection, Southern blotting, qPCR, or microarray analysis
SKU List: NKP107-384
Product Data Sheet
Product Manual